GK069 ScienCell 人类小胶质细胞生物学qPCR检测试剂盒，Human Microglia Cell Biology qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京作为ScienCell品牌的代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK069 ScienCell人类小胶质细胞生物学qPCR检测试剂盒，Human Microglia Cell Biology qPCR Array Kit，产品简介：

产品品牌： ScienCell

产品货号：GK069

产品名称：GK069 ScienCell人类小胶质细胞生物学qPCR检测试剂盒，Human Microglia Cell Biology qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

GK069 ScienCell人类小胶质细胞生物学qPCR检测试剂盒，Human Microglia Cell Biology qPCR Array Kit， 产品说明：

ScienCell 的 GeneQuery™ 人类小胶质细胞生物学 qPCR 阵列 （GQH-MGL） 有助于对参与小胶质细胞调节和功能的 88 个关键基因进行基因表达分析。 小胶质细胞被认为是中枢神经系统（CNS）的主要防御细胞。 虽然小胶质细胞经常被吹捧为“中枢神经系统的常驻巨噬细胞"，但在调节和特殊作用方面与巨噬细胞区分开来。 如何根据其功能对所包含的基因进行分组的示例如下所示：

经典启动： BIRC3， IFNG， IL12A， CXCL10， TYMP

替代启动：气体7、IGF1、CXCL2、SPP1、MRC1

小胶质细胞标志物：PTPRC、ITGAX、AIF1、CD40、EDA

Sensome： GPR34， CXCR2， ADORA3， LTF， SIGLECH

受体：MARCO，MSR1，P2RX4，SIRPA，CSF1R

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有说明，否则引物集可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Microglia Cell Biology qPCR Array (GQH-MGL) facilitates gene expression profiling of 88 key genes involved in microglial regulation and function. Microglia are considered to be the main cells of defense in the central nervous system (CNS). Though often touted as “the resident macrophages of the CNS," microglia distinguish themselves from macrophages in regulation and through specialized roles. Examples of how included genes may be grouped according to their function are shown below:

· Classical priming: BIRC3, IFNG, IL12A, CXCL10, TYMP

· Alternative priming: GAS7, IGF1, CXCL2, SPP1, MRC1

· Microglia markers: PTPRC, ITGAX, AIF1, CD40, EDA

· Sensome: GPR34, CXCR2, ADORA3, LTF, SIGLECH

· Receptors: MARCO, MSR1, P2RX4, SIRPA, CSF1R

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类小胶质细胞生物学 qPCR 阵列板布局*（8 个粗体和斜体对照）

提示：基因选择可能会根据新的研究和开发进行更新

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