产品介绍 抗体类型 一抗同型对照：Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor 450浓度：0.2 mg/mL用法：1 μg/test (Flow)；产品详细信息Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligandsand their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.数据CD279 (PD-1) Antibody (48-9981-80) in FlowStaining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82andProduct # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.5 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (blue histogram) or 0.5 µg of Anti-Mouse CD279 (PD-1) eFluor® 450 (purple histogram). Total viable cells, as determined by 7-AAD (Product # 00-6993-50), were used for analysis.推荐产品：二抗荧光二抗免疫组化一抗标签抗体参考文献：1.The Journal of experimental medicineCreatine uptake regulates CD8 T cell antitumor immunity."Published figure using CD279 (PD-1) monoclonal antibody (Product # 48-9981-82) in Flow Cytometry"2.Nature communicationsInterdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy."Published figure using CD279 (PD-1) monoclonal antibody (Product # 48-9981-82) in Flow Cytometry"3.Nature communicationsIntestinal non-canonical NFκB signaling shapes the local and systemic immune response.AuthorsRamakrishnan SK,Zhang H,Ma X,Jung I,Schwartz AJ,Triner D,Devenport SN,Das NK,Xue X,Zeng MY,Hu Y,Mortensen RM,Greenson JK,Cascalho M,Wobus CE,Colacino JA,Nunez G,Rui L,Shah YM4.Cancer cellFc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies."48-9981 was used in Flow cytometry/Cell sorting to examine the importance of Fc-gamma receptor binding in contributing to the activity of anti-CTLA4 antibodies."5.Nature communicationsInterdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy."Published figure using CD279 (PD-1) monoclonal antibody (Product # 48-9981-82) in Flow Cytometry"

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